Dynamics of individual Brownian rods in a microchannel flow

We study the orientational dynamics of heavy silica microrods flowing through a microfluidic channel. Comparing experiments and Brownian dynamics simulations we identify different particle orbits, in particular in-plane tumbling behavior, which cannot be explained by classical Jeffery theory, and we relate this behavior to the rotational diffusion of the rods. By constructing the full, three-dimensional, orientation distribution, we describe the rod trajectories and quantify the persistence of Jeffery orbits using temporal correlation functions of the Jeffery constant. We find that our colloidal rods lose memory of their initial configuration in about a second, corresponding to half a Jeffery period.Comment: 5 pages, 4 figure

Oscillatory surface rheotaxis of swimming E. coli bacteria

Bacterial contamination of biological conducts, catheters or water resources is a major threat to public health and can be amplified by the ability of bacteria to swim upstream. The mechanisms of this rheotaxis, the reorientation with respect to flow gradients, often in complex and confined environments, are still poorly understood. Here, we follow individual E. coli bacteria swimming at surfaces under shear flow with two complementary experimental assays, based on 3D Lagrangian tracking and fluorescent flagellar labelling and we develop a theoretical model for their rheotactic motion. Three transitions are identified with increasing shear rate: Above a first critical shear rate, bacteria shift to swimming upstream. After a second threshold, we report the discovery of an oscillatory rheotaxis. Beyond a third transition, we further observe coexistence of rheotaxis along the positive and negative vorticity directions. A full theoretical analysis explains these regimes and predicts the corresponding critical shear rates. The predicted transitions as well as the oscillation dynamics are in good agreement with experimental observations. Our results shed new light on bacterial transport and reveal new strategies for contamination prevention.Comment: 12 pages, 5 figure

Periodic and Quasiperiodic Motion of an Elongated Microswimmer in Poiseuille Flow

We study the dynamics of a prolate spheroidal microswimmer in Poiseuille flow for different flow geometries. When moving between two parallel plates or in a cylindrical microchannel, the swimmer performs either periodic swinging or periodic tumbling motion. Although the trajectories of spherical and elongated swimmers are qualitatively similar, the swinging and tumbling frequency strongly depends on the aspect ratio of the swimmer. In channels with reduced symmetry the swimmers perform quasiperiodic motion which we demonstrate explicitely for swimming in a channel with elliptical cross section

Enhanced sedimentation of elongated plankton in simple flows

Negatively buoyant phytoplankton play an important role in the sequestration of CO_2 from the atmo-sphere and are fundamental to the health of the world’s fisheries. However, there is still much to discoveron transport mechanisms from the upper photosynthetic regions to the deep ocean. In contrast to intuitive expectations that mixing increases plankton residence time in light-rich regions, recent experimental and computational evidence suggests that turbulence can actually enhance sedimentation of negatively buoyant diatoms. Motivated by these studies we dissect the enhanced sedimentation mechanisms using the simplest possible two-dimensional flows, avoiding expensive computations and obfuscation. In particular, we find that in vertical shear, preferential flow alignment and aggregation in down-welling regions both increase sedimentation, whereas horizontal shear reduces the sedimentation due only to alignment. However the magnitude of the shear does not affect the sedimentation rate. In simple vertical Kolmogorov flow elongated particles also have an enhanced sedimentation speed as they spend more time in down-welling regions of the flow with vertically aligned orientation, an effect that increases with the magnitude of shear. An additional feature is identified in horizontal Kolomogorov flow, whereby the impact of shear-dependent sedimentation speed is to cause aggregation in regions of high-shear where the sedimentation speed is minimum. In cellular flow, there is an increase in mean sedimentation speed with aspect ratio and shear strength associated with aggregation in down-welling regions. Furthermore, spatially projected trajectories can intersect and give rise to chaotic dynamics, which is associated with a depletion of particles within so called retention zones

Experimental observation of flow fields around active Janus spheres

The phoretic mechanisms at stake in the propulsion of asymmetric colloids have been the subject of debates during the past years. In particular, the importance of electrokinetic effects on the motility of Pt-PS Janus sphere was recently discussed. Here, we probe the hydrodynamic flow field around a catalytically active colloid using particle tracking velocimetry both in the freely swimming state and when kept stationary with an external force. Our measurements provide information about the fluid velocity in the vicinity of the surface of the colloid, and confirm a mechanism for propulsion that was proposed recently. In addition to offering a unified understanding of the nonequilibrium interfacial transport processes at stake, our results open the way to a thorough description of the hydrodynamic interactions between such active particles and understanding their collective dynamics

Driven spheres, ellipsoids and rods in explicitly modeled polymer solutions

Understanding the transport of driven nano- and micro-particles in complex fluids is of relevance for many biological and technological applications. Here we perform hydrodynamic multiparticle collision dynamics simulations of spherical and elongated particles driven through polymeric fluids containing different concentrations of polymers. We determine the mean particle velocities which are larger than expected from Stokes law for all particle shapes and polymer densities. Furthermore we measure the fluid flow fields and local polymer density and polymer conformation around the particles. We find that polymer-depleted regions close to the particles are responsible for an apparent tangential slip velocity which accounts for the measured flow fields and transport velocities. A simple two-layer fluid model gives a good match to the simulation results

Enhanced bacterial swimming speeds in macromolecular polymer solutions

The locomotion of swimming bacteria in simple Newtonian fluids can successfully be described within the framework of low-Reynolds-number hydrodynamics1. The presence of polymers in biofluids generally increases the viscosity, which is expected to lead to slower swimming for a constant bacterial motor torque. Surprisingly, however, experiments have shown that bacterial speeds can increase in polymeric fluids2,3,4,5. Whereas, for example, artificial helical microswimmers in shear-thinning fluids6 or swimming Caenorhabditis elegans worms in wet granular media7,8 increase their speeds substantially, swimming Escherichia coli bacteria in polymeric fluids show just a small increase in speed at low polymer concentrations, followed by a decrease at higher concentrations2,4. The mechanisms behind this behaviour are currently unclear, and therefore we perform extensive coarse-grained simulations of a bacterium swimming in explicitly modelled solutions of macromolecular polymers of different lengths and densities. We observe an increase of up to 60% in swimming speed with polymer density and demonstrate that this is due to a non-uniform distribution of polymers in the vicinity of the bacterium, leading to an apparent slip. However, this in itself cannot predict the large increase in swimming velocity: coupling to the chirality of the bacterial flagellum is also necessary

Enhanced bacterial swimming speeds in macromolecular polymer solutions

The locomotion of swimming bacteria in simple Newtonian fluids can successfully be described within the framework of low-Reynolds-number hydrodynamics1. The presence of polymers in biofluids generally increases the viscosity, which is expected to lead to slower swimming for a constant bacterial motor torque. Surprisingly, however, experiments have shown that bacterial speeds can increase in polymeric fluids2,3,4,5. Whereas, for example, artificial helical microswimmers in shear-thinning fluids6 or swimming Caenorhabditis elegans worms in wet granular media7,8 increase their speeds substantially, swimming Escherichia coli bacteria in polymeric fluids show just a small increase in speed at low polymer concentrations, followed by a decrease at higher concentrations2,4. The mechanisms behind this behaviour are currently unclear, and therefore we perform extensive coarse-grained simulations of a bacterium swimming in explicitly modelled solutions of macromolecular polymers of different lengths and densities. We observe an increase of up to 60% in swimming speed with polymer density and demonstrate that this is due to a non-uniform distribution of polymers in the vicinity of the bacterium, leading to an apparent slip. However, this in itself cannot predict the large increase in swimming velocity: coupling to the chirality of the bacterial flagellum is also necessary